GFP anticorps

N° du produit ABIN7595316

L’anticorps Cobaye Polyclonal anti-GFP a été validé pour WB et IHC. Il convient pour détecter GFP dans des échantillons de Aequorea victoria.

Aperçu rapide pour GFP anticorps (ABIN7595316)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-GFP anticorps

Fonction: Guinea pig antibody to EGFP/GFP/Venus

Specificité: Specific for EGFP/GFP/Venus

Réactivité croisée (Details): Any organism expressing EGFP/GFP/Venus.

Purification: IgG ammonium sulfate cut

Immunogène: Recombinant EGFP/GFP/Venus protein was used as the antigen.

Isotype: IgG

Information d'application

Indications d'application: IHC WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 100 µL of DI sterile water. Centrifuge to remove any insoluble material.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Date de péremption: 12 months

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: EGFP/GFP/Venus

Sujet: GFP is a naturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin's blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer reporter of gene expression or as a measure of protein-protein interactions.